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KMID : 0900919940180040251
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Korean journal of Animal Reproduction
1994 Volume.18 No. 4 p.251 ~ p.256
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Studies on Vitrification of Bovine Blastocysts Fertilized In Vitro
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Lee Myung -Shik
Baek Kwang-Soo
Seong Hwan-Hoo
Oh S.-J.
Yang B.-S.
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Abstract
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Two experiments were conducted to study the production of in vitro fertilized bovine embryos and the viability of blastocysts cryopreserved by vitrification. In experiment 1, production rate of in vitro matured bovine oocytes after fertilization in medium containing bovine oviduct epithelial cells (BOEC), cumulus cells and granulosa cells to blastocysts were 18.4, 14.6 and 13.1%, respectively. Developmental percentages of blastocysts produced at day 6, 7 and 8 were 8.5, 10.6 and 15.2% respectively. Hatching rate of bovine embryos produced was 60.0%. In experiment 2, post-thawed surviving embryos in a vitrification solution consisting of 7.15M ethylene glycol, 2.5 mM ficoll and 0.3 M sucrose were 36.4% (56/154). Also, survival rate of bovine embryos after exposed to vitrification solution at 1, 2, 3, 4 and 5 min were 84.0, 88.0, 71.0, 48.0 and 24.0% respectively.
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KEYWORD
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bovine, in vitro fertilization, embryo, vitrification
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